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Anti-Flag Tag Antibody-FITC (3S989)

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Catalog No. TMAS-00123F
Alias flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody-FITC (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-FITC (3S989) can be used in FCM, ICC/IF.

Anti-Flag Tag Antibody-FITC (3S989)

Anti-Flag Tag Antibody-FITC (3S989)

😃Good
Catalog No. TMAS-00123FAlias flagtag, DYKDDDDK-tag
Anti-Flag Tag Antibody-FITC (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-FITC (3S989) can be used in FCM, ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
40 μg$6097-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Flag Tag Antibody-FITC (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-FITC (3S989) can be used in FCM, ICC/IF.
Synonymsflagtag, DYKDDDDK-tag
Ig Type
IgG
Clone
3S989
Specificity
DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins
Verified Activity
1. Flow cytometric analysis of CHO/Plasmid cells (Red) and CHO negative control cell (Black) binding with Anti-Flag Tag Antibody-FITC (3S989) (TMAS-00123F).
Antibody working concentration: 5 µg/ml, 2.5x10⁵ cells/reaction.
2. Immunofluorescence staining of Anti-Flag Tag Antibody-FITC (3S989) (TMAS-00123F) with DYKDDDDK Tag in CHO-K1 cells. CHO-K1 cells transfected with pcDNA3.1(+) (DYKDDDDK Tag-RFP) (Red) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min.The cells were then incubated with 1/100 Anti-Flag Tag Antibody-FITC (3S989) (TMAS-00123F) (Green) at 37℃ for 1h.
Application
Recommended Dose
FCM: 1 μg/ml; ICC/IF: 1 μg/ml
Antibody Type
Monoclonal
Host SpeciesRabbit
PurificationProtein A affinity column
AppearanceLyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
FormulationLyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Research Background0
Related Conjugates and Formulations
Conjucates
FITC
Others Formats
Unconjugated/HRP/Biotin
Antibody Types Available
4
Antigen Details
Immunogen
KLH conjugated synthetic peptide: DYKDDDDK
Chemical Properties
Stability & Storage
Stability & StorageThe lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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