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Anti-Flag Tag Antibody-FITC (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-FITC (3S989) can be used in FCM, ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 40 μg | $609 | 7-10 days | 7-10 days |
| Description | Anti-Flag Tag Antibody-FITC (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-FITC (3S989) can be used in FCM, ICC/IF. |
| Synonyms | flagtag, DYKDDDDK-tag |
| Ig Type | IgG |
| Clone | 3S989 |
| Specificity | DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins |
| Verified Activity | 1. Flow cytometric analysis of CHO/Plasmid cells (Red) and CHO negative control cell (Black) binding with Anti-Flag Tag Antibody-FITC (3S989) (TMAS-00123F). Antibody working concentration: 5 µg/ml, 2.5x10⁵ cells/reaction. 2. Immunofluorescence staining of Anti-Flag Tag Antibody-FITC (3S989) (TMAS-00123F) with DYKDDDDK Tag in CHO-K1 cells. CHO-K1 cells transfected with pcDNA3.1(+) (DYKDDDDK Tag-RFP) (Red) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min.The cells were then incubated with 1/100 Anti-Flag Tag Antibody-FITC (3S989) (TMAS-00123F) (Green) at 37℃ for 1h. |
| Application | |
| Recommended Dose | FCM: 1 μg/ml; ICC/IF: 1 μg/ml |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Purification | Protein A affinity column |
| Appearance | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Formulation | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. |
| Research Background | 0 |
| Conjucates | FITC |
| Others Formats | Unconjugated/HRP/Biotin |
| Antibody Types Available | 4 |
| Immunogen | KLH conjugated synthetic peptide: DYKDDDDK |
| Stability & Storage | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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