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Anti-Flag Tag Antibody (7J897)
😃Good
Catalog No. TMAC-01502
Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in IF,IP,WB.
Anti-Flag Tag Antibody (7J897)
Anti-Flag Tag Antibody (7J897)
😃Good
Catalog No. TMAC-01502
Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in IF,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Chemical Properties
| Description | Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in IF,IP,WB. |
| Clone | 7J897 |
| Verified Activity | 1. 1ug Flag fusion protein+ Primary antibody TMAC-01502 dilution at 1:10,000.
2. IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb TMAC-01502 at a 1:2000 dilution (blue DAPI, red anti-Flag). 3. IP antibody use 5ug Flag Mouse IgG1 per ml Lysate WB 1:5000, 1 untransfected 293 cell lysate, 2 transfected 293 cell lysate with Flag-tag fusion protein, 3 IP (transfected 293+ normal Mouse IgG+Protein G agarose, 4 IP (transfected 293+anti- Flag mAb+ Protein G agarose), 5 IP (transfected 293+Protein G), 6 Recombinant protein (E.coli).    |
| Application | |
| Recommended Dose | WB: 1:5000-10000; IF: 1:2000; IP: 1:5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Affinity purification using immunogen. |
| Appearance | Liquid |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
| Conjucates | Unconjugated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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