Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Flag Tag Antibody (7J897)
😃Good
Catalog No. TMAC-01502
Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in WB,IF,IP.
Anti-Flag Tag Antibody (7J897)
Anti-Flag Tag Antibody (7J897)
😃Good
Catalog No. TMAC-01502
Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in WB,IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $216 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View MoreResource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Chemical Properties
| Description | Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in WB,IF,IP. |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 1ug Flag fusion protein+ Primary antibody TMAC-01502 dilution at 1:10,000. 2. IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb TMAC-01502 at a 1:2000 dilution (blue DAPI, red anti-Flag). 3. IP antibody use 5ug Flag Mouse IgG1 per ml Lysate WB 1:5000, 1 untransfected 293 cell lysate, 2 transfected 293 cell lysate with Flag-tag fusion protein, 3 IP (transfected 293+ normal Mouse IgG+Protein G agarose, 4 IP (transfected 293+anti- Flag mAb+ Protein G agarose), 5 IP (transfected 293+Protein G), 6 Recombinant protein (E.coli). |
| Application | |
| Recommended Dose | WB: 1:5000-10000; IF: 1:2000; IP: 1:5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Affinity purification using immunogen. |
| Appearance | Liquid |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
| Conjucates | Unconjugated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-Flag Tag Antibody (7J897) | purchase Anti-Flag Tag Antibody (7J897) | Anti-Flag Tag Antibody (7J897) cost | order Anti-Flag Tag Antibody (7J897)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.