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Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $216 | 7-10 days | |
100 μL | $317 | 7-10 days |
Description | Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB. |
Application | |
Recommended Dose | WB: 1:5000-10000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | Affinity purification using immunogen. |
Appearance | Liquid |
Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
Conjucates | Unconjugated |
Immunogen | T524 |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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