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Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
| Description | Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB. |
| Clone | 2K280 |
| Verified Activity | 1. Western blot analysis of Recombinant protein, using TMAC-01503 diluted at 1) 1:5,000, 2) 1:10,000. ![]() |
| Application | |
| Recommended Dose | WB: 1:5000-10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Affinity purification using immunogen. |
| Appearance | Liquid |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
| Conjucates | Unconjugated |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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