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Anti-Flag Tag Antibody (2K280)

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Catalog No. TMAC-01503 Copy Product Info
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Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB.

Anti-Flag Tag Antibody
(2K280)

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2167-10 days7-10 days
100 µL$3177-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB.
Clone
2K280
Verified Activity
1. Western blot analysis of Recombinant protein, using TMAC-01503 diluted at 1) 1:5,000, 2) 1:10,000.
Anti-Flag Tag Antibody (2K280)
Application
Recommended Dose
WB: 1:5000-10000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationAffinity purification using immunogen.
AppearanceLiquid
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundThe DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Related Conjugates and Formulations
Conjucates
Unconjugated
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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