Anti-FITM1 Polyclonal Antibody is a Rabbit antibody targeting FITM1. Anti-FITM1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30 min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (TMAB-06038) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30 min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (TMAB-06038) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30 min; Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (TMAB-06038) at 1:400 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.
4. Protein: 293T (human) lysate at 40 μg;
Primary: rabbit Anti-FITM1 (TMAB-06038) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
Predicted band size: 32 kD
Observed band size: 28 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human FITM1

Human

FITM1

Fat storage-inducing transmembrane protein 1

Plays an important role in lipid droplet accumulation.