Anti-FIS1 Antibody (8U616) is a Mouse antibody targeting FIS1. Anti-FIS1 Antibody (8U616) can be used in WB,ICC/IF.

IgG1, Kappa

8U616

Human

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2 h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
2. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (TMAB-06035) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

KLH conjugated synthetic peptide: human FIS1

Human

FIS1

Mitochondrial fission 1 protein

Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.