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Anti-Filaggrin Polyclonal Antibody
😃Good
Catalog No. TMAB-00680
Alias FLG, Filaggrin
Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Filaggrin Polyclonal Antibody
Anti-Filaggrin Polyclonal Antibody
😃Good
Catalog No. TMAB-00680Alias FLG, Filaggrin
Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | FLG, Filaggrin |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]. |
| Immunogen | KLH conjugated synthetic peptide: human Filaggrin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epi / Endo-thelial,Cytokeratin,Cytokeratins |
| Molecular Weight | Theoretical: 447 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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