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Anti-Filaggrin Polyclonal Antibody

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Catalog No. TMAB-00680

Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Filaggrin Polyclonal Antibody

Anti-Filaggrin Polyclonal Antibody

😃Good
Catalog No. TMAB-00680
Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Filaggrin
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Epi / Endo-thelial,Cytokeratin,Cytokeratins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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