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Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | IHC-P,IHC-Fr,IF |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]. |
Immunogen | KLH conjugated synthetic peptide: human Filaggrin |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Epi / Endo-thelial,Cytokeratin,Cytokeratins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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