Anti-Fibrinogen alpha chain Antibody (5J106) is a Rabbit antibody targeting Fibrinogen alpha chain. Anti-Fibrinogen alpha chain Antibody (5J106) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

5J106

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibrinogen alpha chain Monoclonal Antibody, Unconjugated (TMAB-06022) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibrinogen alpha chain Monoclonal Antibody, Unconjugated (TMAB-06022) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibrinogen alpha chain Monoclonal Antibody, Unconjugated (TMAB-06022) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibrinogen alpha chain Monoclonal Antibody, Unconjugated (TMAB-06022) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibrinogen alpha chain Monoclonal Antibody, Unconjugated (TMAB-06022) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibrinogen alpha chain Monoclonal Antibody, Unconjugated (TMAB-06022) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. 25 μg total protein per lane of various lysates (see on figure) probed with Fibrinogen alpha chain monoclonal antibody, unconjugated (TMAB-06022) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
8. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Fibrinogen alpha chain antibody (TMAB-06022, 1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human Fibrinogen alpha chain

Human

FGA/FGB/FGG

Fibrinogen alpha chain

Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.