Anti-Fibrillarin Antibody (3P424) is a Rabbit antibody targeting Fibrillarin. Anti-Fibrillarin Antibody (3P424) can be used in WB,ICC/IF,FCM.

IgG/Kappa

3P424

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with Fibrillarin monoclonal antibody, unconjugated (TMAB-06019) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-FBL antibody (TMAB-06019, 1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (FBL) monoclonal Antibody, unconjugated (TMAB-06019) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

FBL

rRNA 2'-O-methyltransferase fibrillarin