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Anti-FENS1 Polyclonal Antibody
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Catalog No. TMAB-05962
Anti-FENS1 Polyclonal Antibody is a Rabbit antibody targeting FENS1. Anti-FENS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-FENS1 Polyclonal Antibody
Anti-FENS1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-05962
Anti-FENS1 Polyclonal Antibody is a Rabbit antibody targeting FENS1. Anti-FENS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-FENS1 Polyclonal Antibody is a Rabbit antibody targeting FENS1. Anti-FENS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FENS1) Polyclonal Antibody, Unconjugated (TMAB-05962) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: U87MG (Human) Cell Lysate at 30 μg Lane 2: HepG2 (Human) Cell Lysate at 30 μg Lane 3: U2os (Human) Cell Lysate at 30 μg Lane 4: A431 (Human) Cell Lysate at 30 μg Primary: Anti-FENS1 (TMAB-05962) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD 3. Sample: Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 μg Lane 2: Cerebrum (Rat) Tissue Lysate at 40 μg Lane 3: Skin (Rat) Tissue Lysate at 40 μg Lane 4: Huvec (Human) Cell Lysate at 30 μg Lane 5: HepG2 (Human) Cell Lysate at 30 μg Lane 6: THP-1 (Human) Cell Lysate at 30 μg Primary: Anti-FENS1 (TMAB-05962) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 48 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Early endosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. WDFY1 (WD repeat and FYVE domain containing 1), also known as WDF1, FENS-1 or ZFYVE17, is a 410 amino acid protein that localizes to the early endosome and contains one FYVE-type zinc finger and seven WD repeats through which it may play a role in protein trafficking and signal transduction. |
| Immunogen | KLH conjugated synthetic peptide: human FENS1 |
| Antigen Species | Human |
| Gene Name | WDFY1 |
| Gene ID | |
| Protein Name | WD repeat and FYVE domain-containing protein 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 46 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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