Anti-FCGR1A/CD64 Polyclonal Antibody 2 is a Rabbit antibody targeting FCGR1A/CD64. Anti-FCGR1A/CD64 Polyclonal Antibody 2 can be used in FCM.

IgG

Mouse,Human

1. Blank control (black line): Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD64 antibody (TMAB-05945)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. The THP-1 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-FCGR1A/CD64 antibody (TMAB-05945): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

Recombinant Protein: mouse FCGR1A (CD64) protein

Mouse

FCGR1A

High affinity immunoglobulin gamma Fc receptor I