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Anti-FBXO45 Polyclonal Antibody

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Catalog No. TMAB-00663
Alias hFbxo45, fbxo45, FBX45, FBSP1, F-box/SPRY domain-containing protein 1, F box protein 45

Anti-FBXO45 Polyclonal Antibody is a Rabbit antibody targeting FBXO45. Anti-FBXO45 Polyclonal Antibody can be used in WB.

Anti-FBXO45 Polyclonal Antibody

Anti-FBXO45 Polyclonal Antibody

😃Good
Catalog No. TMAB-00663Alias hFbxo45, fbxo45, FBX45, FBSP1, F-box/SPRY domain-containing protein 1, F box protein 45
Anti-FBXO45 Polyclonal Antibody is a Rabbit antibody targeting FBXO45. Anti-FBXO45 Polyclonal Antibody can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
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Product Introduction

Bioactivity
Description
Anti-FBXO45 Polyclonal Antibody is a Rabbit antibody targeting FBXO45. Anti-FBXO45 Polyclonal Antibody can be used in WB.
AliashFbxo45, fbxo45, FBX45, FBSP1, F-box/SPRY domain-containing protein 1, F box protein 45
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Intestine (Mouse) Lysate at 40 μg
Primary: Anti-FBXO45 (TMAB-00663) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
2. Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-FBXO45 (TMAB-00663) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 30 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > presynaptic cell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundBelonging to the F-box family of proteins, FBXO45 is a 286 amino acid protein that contains one C-terminal F-box domain. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein)-type E3 ubiquitin ligase complex and are involved in substrate recognition and protein recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular mechanisms, including the cell cycle, the immune response, signaling cascades and developmental processes. They function by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, I˚B-å and ∫-catenin, for degradation by the proteasome after ubiquitination. Via its F-box domain, FBXO45 can directly interact with Skp1 p19 and CUL-1. FBXO45 has been shown to be an estrogen-induced gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FBXO45
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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