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Anti-FASN Antibody (1K266)
😃Good
Catalog No. TMAB-00658
Alias SDR27X1, OA-519, fatty acid synthase, FAS
Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-FASN Antibody (1K266)
Anti-FASN Antibody (1K266)
😃Good
Catalog No. TMAB-00658Alias SDR27X1, OA-519, fatty acid synthase, FAS
Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | SDR27X1, OA-519, fatty acid synthase, FAS |
| Ig Type | IgG1κ |
| Clone | 1K266 |
| Reactivity | Human (predicted:Mouse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 5. 25 μg total protein per Lane of various lysates probed with FASN monoclonal antibody, unconjugated (TMAB-00658) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:20-200; ICC/IF: 1:100-500; IF: 1:20-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Affinity purified by Protein AGL |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. |
| Immunogen | KLH conjugated synthetic peptide: human FASN |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism |
| Molecular Weight | Theoretical: 274 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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