Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG1κ

1K266

Human (predicted:Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. 25 μg total protein per Lane of various lysates probed with FASN monoclonal antibody, unconjugated (TMAB-00658) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:20-200; ICC/IF: 1:100-500; IF: 1:20-200

Monoclonal

KLH conjugated synthetic peptide: human FASN

Human

FASN

Fatty acid synthase

Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.