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Anti-FASN Antibody (1K266)

😃Good
Catalog No. TMAB-00658
Alias SDR27X1, OA-519, fatty acid synthase, FAS

Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-FASN Antibody (1K266)

Anti-FASN Antibody (1K266)

😃Good
Catalog No. TMAB-00658Alias SDR27X1, OA-519, fatty acid synthase, FAS
Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4897-10 days
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Product Introduction

Bioactivity
Description
Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
AliasSDR27X1, OA-519, fatty acid synthase, FAS
Ig Type
IgG1κ
Clone
1K266
Reactivity
Human (predicted:Mouse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. 25 μg total protein per Lane of various lysates probed with FASN monoclonal antibody, unconjugated (TMAB-00658) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:20-200; ICC/IF: 1:100-500; IF: 1:20-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ConstructionHybridoma Monoclonal Antibody
PurificationAffinity purified by Protein AGL
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundFASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FASN
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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