Anti-FAIM3 Polyclonal Antibody 2 is a Rabbit antibody targeting FAIM3. Anti-FAIM3 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-FAIM3 Polyclonal Antibody, Unconjugated (TMAB-05806) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-FAIM3 Polyclonal Antibody, Unconjugated (TMAB-05806) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-FAIM3 (TMAB-05806) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 31 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human FAIM3

Human

FCMR

Fas apoptotic inhibitory molecule 3

May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation.