Anti-FAF2 Antibody (7W565) is a Mouse antibody targeting FAF2. Anti-FAF2 Antibody (7W565) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG2b/Kappa

7W565

Human (predicted:Mouse,Rat)

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2 h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: 293, 2: HepG2, 3: Mouse lung, 4: Rat liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 51 kDa
Observed MW: 55 kDa
2. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-05805
3. Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-05805

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:100-500; IF: 1:50-200

Monoclonal

FAF2

FAS-associated factor 2

May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.