Anti-FADD Antibody (5V608) is a Rabbit antibody targeting FADD. Anti-FADD Antibody (5V608) can be used in WB,IHC-P,IHC-Fr,FCM,IF.

IgG

5V608

Mouse

1. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FADD Monoclonal Antibody, Unconjugated (TMAB-05801) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. 25 μg total protein per lane of various lysates (see on figure) probed with FADD monoclonal antibody, unconjugated (TMAB-05801) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; FCM: 1:50-100; IF: 1:50-200

Monoclonal

A synthesized peptide: mouse Fadd

Mouse

FADD

FAS-associated death domain protein

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.