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Anti-factor VIII Polyclonal Antibody

Catalog No. TMAB-05794 Copy Product Info
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Anti-factor VIII Polyclonal Antibody is a Rabbit antibody targeting factor VIII. Anti-factor VIII Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-factor VIII Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-05794

Anti-factor VIII Polyclonal Antibody is a Rabbit antibody targeting factor VIII. Anti-factor VIII Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-factor VIII Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-factor VIII Polyclonal Antibody is a Rabbit antibody targeting factor VIII. Anti-factor VIII Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37??for 20 min;
Incubation: Anti-factor VIIi (FVIII) (human) Polyclonal Antibody, Unconjugated (TMAB-05794) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
IHC-P
IHC-Fr
IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human factor VIII
Antigen Species
Human
Gene Name
F8
Gene ID
2157
Protein Name
Coagulation factor VIII
Uniprot ID
P00451
Function
Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Chemical Properties
Molecular WeightTheoretical: 230 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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