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Anti-Ezrin Antibody (7C964)
😃Good
Catalog No. TMAY-02424
Alias VIL2, HEL-S-105, ezrin, CVL, CVIL
Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in WB,IP.
Anti-Ezrin Antibody (7C964)
Anti-Ezrin Antibody (7C964)
😃Good
Catalog No. TMAY-02424Alias VIL2, HEL-S-105, ezrin, CVL, CVIL
Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IP Reactivity: Human |
| Alias | VIL2, HEL-S-105, ezrin, CVL, CVIL |
| Ig Type | Mouse IgG1 |
| Clone | 7C964 |
| Reactivity | Human |
| Verified Activity | 1. Anti-EZR mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:69 kDa. -Observed band size:90 kDa(We are unsure as to the identity of these extra bands.) 2. EZR was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate -0.5 µL anti-EZR mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-EZR mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 69 kDa. -Observed band size: 90 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:1000; IP: 0.1-0.5 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ezrin / villin 2 / EZR (rh Ezrin / villin 2 / EZR; TMPY-03053; P15311; Pro2-Leu586). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | VIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskel |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Ezrin / villin 2 / EZR Protein (TMPY-03053) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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