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Anti-Ezrin Antibody (7C964)

😃Good
Catalog No. TMAY-02424
Alias VIL2, HEL-S-105, ezrin, CVL, CVIL

Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in WB,IP.

Anti-Ezrin Antibody (7C964)

Anti-Ezrin Antibody (7C964)

😃Good
Catalog No. TMAY-02424Alias VIL2, HEL-S-105, ezrin, CVL, CVIL
Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in WB,IP.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in WB,IP.
AliasVIL2, HEL-S-105, ezrin, CVL, CVIL
Ig Type
Monoclonal Mouse IgG1
Clone
7C964
Reactivity
Human
Verified Activity
1. Anti-EZR mouse monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: Jurkat Whole Cell Lysate.

-Lane C: A431 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:69 kDa.

-Observed band size:90 kDa(We are unsure as to the identity of these extra bands.)

2. EZR was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg HepG2 Whole Cell Lysate

-0.5 µL anti-EZR mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

-Primary antibody:

-Anti-EZR mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 69 kDa.

-Observed band size: 90 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:1000; IP: 0.1-0.5 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ezrin / villin 2 / EZR (rh Ezrin / villin 2 / EZR; TMPY-03053; P15311; Pro2-Leu586). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundVIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskel
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human Ezrin / villin 2 / EZR Protein (TMPY-03053)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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