Shopping Cart
Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-Ezrin Antibody (7C964) (Synonyms: VIL2, HEL-S-105, ezrin, CVL, CVIL)

Catalog No. TMAY-02424 Copy Product Info
🥰Excellent
Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in IP,WB.

Anti-Ezrin Antibody (7C964)

Copy Product Info
🥰Excellent
Catalog No. TMAY-02424
Synonyms VIL2, HEL-S-105, ezrin, CVL, CVIL

Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in IP,WB.

Anti-Ezrin Antibody
(7C964)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$1987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
Add to Cart
Add to Quotation
For research use only—not for human use. No sales to individuals. Use as intended only.
Questions
TargetMol
View More

Product Introduction

Bioactivity
Description
Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in IP,WB.
SynonymsVIL2, HEL-S-105, ezrin, CVL, CVIL
Ig Type
Mouse IgG1
Clone
7C964
Reactivity
Human
Specificity
Human Ezrin
Verified Activity
1. Anti-EZR mouse monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: Jurkat Whole Cell Lysate.
-Lane C: A431 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:69 kDa.
-Observed band size:90 kDa(We are unsure as to the identity of these extra bands.)
2. EZR was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate
-0.5 µL anti-EZR mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
-Primary antibody:
-Anti-EZR mouse monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 69 kDa.
-Observed band size: 90 kDa
Application
Recommended Dose
WB: 1:500-1:1000; IP: 0.1-0.5 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ezrin / villin 2 / EZR (rh Ezrin / villin 2 / EZR; TMPY-03053; P15311; Pro2-Leu586). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundVIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskel
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human Ezrin / villin 2 / EZR Protein (TMPY-03053)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Calculator

  • Dilution Calculator
  • Reconstitution Calculator

Keywords

Related Tags: buy Anti-Ezrin Antibody (7C964) | purchase Anti-Ezrin Antibody (7C964) | Anti-Ezrin Antibody (7C964) cost | order Anti-Ezrin Antibody (7C964)