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Anti-EZH2 Antibody (5A657)
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Catalog No. TMAC-02335
Alias Lysine N-methyltransferase 6, KMT6, Histone-lysine N-methyltransferase EZH2, EZH 2, ENX-1, Enhancer of zeste homolog 2, EC 2.1.1.43
Anti-EZH2 Antibody (5A657) is a Rabbit antibody targeting EZH2. Anti-EZH2 Antibody (5A657) can be used in WB,ICC/IF,IHC.
Anti-EZH2 Antibody (5A657)
Anti-EZH2 Antibody (5A657)
😃Good
Catalog No. TMAC-02335Alias Lysine N-methyltransferase 6, KMT6, Histone-lysine N-methyltransferase EZH2, EZH 2, ENX-1, Enhancer of zeste homolog 2, EC 2.1.1.43
Anti-EZH2 Antibody (5A657) is a Rabbit antibody targeting EZH2. Anti-EZH2 Antibody (5A657) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-EZH2 Antibody (5A657) is a Rabbit antibody targeting EZH2. Anti-EZH2 Antibody (5A657) can be used in WB,ICC/IF,IHC. |
| Synonyms | Lysine N-methyltransferase 6, KMT6, Histone-lysine N-methyltransferase EZH2, EZH 2, ENX-1, Enhancer of zeste homolog 2, EC 2.1.1.43 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of KMT6/EZH2 on different lysates using anti-KMT6/EZH2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: 293. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin. 5. ICC staining KMT6/EZH2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining KMT6/EZH2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining KMT6/EZH2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG), which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. The gene encoding human ENX-1 transcribes a 746 amino acid polypeptide which contains a trithorax-like domain and a DNA-binding motif. ENX-1 interacts with the proto-oncogene product Vav and is thought to be involved in the proliferation of normal and malignant hematopoietic cells. By altering the regulation of target genes, ENX-1 may also contribute to certain phenotypes of Down syndrome. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 86 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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