Anti-ETS1 Polyclonal Antibody is a Rabbit antibody targeting ETS1. Anti-ETS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ETS1 Polyclonal Antibody, Unconjugated (TMAB-05717) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: spleen (Mouse) Lysate at 30 μg
Primary: Anti-ETS1 (TMAB-05717) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
3. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ETS1 antibody (TMAB-05717) Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Thymus tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Lymph node tissue lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human U251 cell lysates
Primary: Anti-ETS1 (TMAB-05717) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Ets1

Human

ETS1

Protein C-ets-1

Transcription factor.