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Anti-ETS1 Antibody (5E990) is a Rabbit antibody targeting ETS1. Anti-ETS1 Antibody (5E990) can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
| Description | Anti-ETS1 Antibody (5E990) is a Rabbit antibody targeting ETS1. Anti-ETS1 Antibody (5E990) can be used in WB,FCM. |
| Ig Type | IgG |
| Clone | 5E990 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ETS1 antibody (TMAB-05718): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 2. 25 μg total protein per lane of various lysates (see on figure) probed with ETS1 monoclonal antibody, unconjugated (TMAB-05718) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]. |
| Immunogen | A synthesized peptide: human ETS1 |
| Antigen Species | Human |
| Gene Name | ETS1 |
| Gene ID | |
| Protein Name | Protein C-ets-1 |
| Uniprot ID | |
| Function | Transcription factor. |
| Molecular Weight | Theoretical: 49 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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