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Anti-ERp57 Polyclonal Antibody
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Catalog No. TMAB-05685
Anti-ERp57 Polyclonal Antibody is a Rabbit antibody targeting ERp57. Anti-ERp57 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ERp57 Polyclonal Antibody
Anti-ERp57 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-05685
Anti-ERp57 Polyclonal Antibody is a Rabbit antibody targeting ERp57. Anti-ERp57 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ERp57 Polyclonal Antibody is a Rabbit antibody targeting ERp57. Anti-ERp57 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Rabbit) |
| Verified Activity | 1. Sample: embryo (Mouse) Lysate at 40 μg Primary: Anti-ERp57 (TMAB-05685) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERp57) Polyclonal Antibody, Unconjugated (TMAB-05685) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 3. Sample: Lane 1: Mouse Skin tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Placenta tissue lysates Lane 4: Rat Testis tissue lysates Lane 5: Human A375 cell lysates Lane 6: Human HepG2 cell lysates Lane 7: Human HeLa cell lysates Primary: Anti-ERp57 (TMAB-05685) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 55 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Tissue Specificity | Detected in the flagellum and head region of spermatozoa (at protein level). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human ERp57 |
| Antigen Species | Human |
| Gene Name | PDIA3 |
| Gene ID | |
| Protein Name | Protein disulfide-isomerase A3 |
| Uniprot ID |
| Molecular Weight | Theoretical: 54 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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