Anti-ERK5 Polyclonal Antibody 2 is a Rabbit antibody targeting ERK5. Anti-ERK5 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Sheep)

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK5) Polyclonal Antibody, Unconjugated (TMAB-05674) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ERK5/MAPK7

Human

MAPK7

Mitogen-activated protein kinase 7

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression.