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Anti-ERK5 Antibody (5E286)
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Catalog No. TMAC-01373
Alias PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen-activated protein kinase 7, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular signal-regulated kinase 5, Extracellular Signal Regulated Kinase 5, ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK1, BMK 1 kinase, BMK 1, Big MAP kinase 1
Anti-ERK5 Antibody (5E286) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (5E286) can be used in WB,ICC/IF,IP,FCM.
Anti-ERK5 Antibody (5E286)
Anti-ERK5 Antibody (5E286)
😃Good
Catalog No. TMAC-01373Alias PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen-activated protein kinase 7, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular signal-regulated kinase 5, Extracellular Signal Regulated Kinase 5, ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK1, BMK 1 kinase, BMK 1, Big MAP kinase 1
Anti-ERK5 Antibody (5E286) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (5E286) can be used in WB,ICC/IF,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-ERK5 Antibody (5E286) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (5E286) can be used in WB,ICC/IF,IP,FCM. |
| Synonyms | PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen-activated protein kinase 7, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular signal-regulated kinase 5, Extracellular Signal Regulated Kinase 5, ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK1, BMK 1 kinase, BMK 1, Big MAP kinase 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution. 2. ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining ERK5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining ERK5 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of A549 cells with ERK5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 115 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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