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Anti-ERK1/2 Polyclonal Antibody
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Catalog No. TMAB-00633
Alias Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1
Anti-ERK1/2 Polyclonal Antibody is a Rabbit antibody targeting ERK1/2. Anti-ERK1/2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-ERK1/2 Polyclonal Antibody
Anti-ERK1/2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00633Alias Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1
Anti-ERK1/2 Polyclonal Antibody is a Rabbit antibody targeting ERK1/2. Anti-ERK1/2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-ERK1/2 Polyclonal Antibody is a Rabbit antibody targeting ERK1/2. Anti-ERK1/2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,Goat) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (TMAB-00633) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Brain (Rat) Lysate at 30 μg Heart (Rat) lysate at 30 μg Primary: Anti-ERK2/MAPK1 (TMAB-00633) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/3000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (TMAB-00633) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Blank control: Hep G2 cells (blue). Primary Antibody: Rabbit Anti-ERK1 + ERK2 antibody (TMAB-00633), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (TMAB-00633, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. 5. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: MCF-7 (Human) Cell Lysate at 30 μg Lane 3: Huvec (Human) Cell Lysate at 30 μg Primary: Anti-ERK1 + ERK2 (TMAB-00633) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44/42 kDa Observed band size: 42/40 kDa 6. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Lane 3: LympHnode (Mouse) Lysate at 40 μg Lane 4: LympHnode (Rat) Lysate at 40 μg Primary: Anti-ERK1 + ERK2 (TMAB-00633) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44/42 kDa Observed band size: 40 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human ERK2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Tangles & Tau,MAPK Pathway,Cytoplasmic |
| Molecular Weight | Theoretical: 42 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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