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Anti-EPX Polyclonal Antibody
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Catalog No. TMAB-00629
Alias PERE, EPX PEN, EPP, EPO, EPER, Eosinophil peroxidase heavy chain, Eosinophil peroxidase
Anti-EPX Polyclonal Antibody is a Rabbit antibody targeting EPX. Anti-EPX Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
Anti-EPX Polyclonal Antibody
Anti-EPX Polyclonal Antibody
😃Good
Catalog No. TMAB-00629Alias PERE, EPX PEN, EPP, EPO, EPER, Eosinophil peroxidase heavy chain, Eosinophil peroxidase
Anti-EPX Polyclonal Antibody is a Rabbit antibody targeting EPX. Anti-EPX Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-EPX Polyclonal Antibody is a Rabbit antibody targeting EPX. Anti-EPX Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P. |
| Synonyms | PERE, EPX PEN, EPP, EPO, EPER, Eosinophil peroxidase heavy chain, Eosinophil peroxidase |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat) |
| Verified Activity | Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EPX Polyclonal Antibody, Unconjugated (TMAB-00629) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.  |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | EPX, eosinophil peroxidase, is an antimycobacterial protein that localizes to cytoplasmic granules of eosinophils and recruits bromide to generate a halogenating oxidant. EPX dependent generation of hypobromous acid causes damage to tissue during inflammatory conditions that include asthma, allergies,cancer and parasitic/helminthic infections. EPX is a major enzyme present in eosinophils and upon degranulation, becomes released into the airways of asthmatics. As a result of its cationic nature and its ability to catalyze the formation of highly toxic oxidants, EPX can induce lung injury in a JNK dependent manner. |
| Immunogen | KLH conjugated synthetic peptide: human Eosinophil peroxidase heavy chain |
| Antigen Species | Human |
| Gene Name | EPX |
| Gene ID | |
| Protein Name | Eosinophil peroxidase |
| Biology Area | Neutrophil Lineage,Cellular metabolic process |
| Function | Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. |
| Molecular Weight | Theoretical: 79 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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