Anti-EPO/Erythropoietin Polyclonal Antibody is a Rabbit antibody targeting EPO/Erythropoietin. Anti-EPO/Erythropoietin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EPO Polyclonal Antibody, Unconjugated (TMAB-00627) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Rat Liver tissue lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-EPO (TMAB-00627) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 33 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Erythropoietin

Human

Response to hypoxia,Growth Factors,Erythrocytes,Hematopoietic system development,ChIP kits,Hypoxia,Hormones,Intracellular Molecules