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Anti-Ephrin B2/EFNB2 Antibody (6Q379)
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Catalog No. TMAB-00626
Alias LERK5, Htk-L, HTKL, EPLG5, ephrin-B2
Anti-Ephrin B2/EFNB2 Antibody (6Q379) is a Rabbit antibody targeting Ephrin B2/EFNB2. Anti-Ephrin B2/EFNB2 Antibody (6Q379) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Ephrin B2/EFNB2 Antibody (6Q379)
Anti-Ephrin B2/EFNB2 Antibody (6Q379)
😃Good
Catalog No. TMAB-00626Alias LERK5, Htk-L, HTKL, EPLG5, ephrin-B2
Anti-Ephrin B2/EFNB2 Antibody (6Q379) is a Rabbit antibody targeting Ephrin B2/EFNB2. Anti-Ephrin B2/EFNB2 Antibody (6Q379) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $273 | 7-10 days | |
| 100 μL | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Ephrin B2/EFNB2 Antibody (6Q379) is a Rabbit antibody targeting Ephrin B2/EFNB2. Anti-Ephrin B2/EFNB2 Antibody (6Q379) can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | LERK5, Htk-L, HTKL, EPLG5, ephrin-B2 |
| Ig Type | IgG |
| Clone | 6Q379 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Lane 3: U251 (Human) Cell Lysate at 30 μg Primary: Anti-Ephrin B2 (TMAB-00626) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45-50 kDa Observed band size: 47 kDa |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Ephrin B2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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