Anti-EphB2 Polyclonal Antibody is a Rabbit antibody targeting EphB2. Anti-EphB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Rat,Mouse,Human (predicted:Dog,Chicken)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated (TMAB-00625) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated (TMAB-00625) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Testis (Rat) Lysate at 40 μg
Lane 3: Huvec (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Lane 5: A431 (Human) Cell Lysate at 30 μg
Lane 6: U2os (Human) Cell Lysate at 30 μg
Lane 7: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 8: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-EphB2 (TMAB-00625) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 120 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human EphB2

Human

Eph Receptors,Growth Cone,Axonal Guidance Proteins,Kinases,Tyrosine Kinase Receptors,Receptor Tyrosine Kinases