Anti-EpCAM Polyclonal Antibody 3 is a Rabbit antibody targeting EpCAM. Anti-EpCAM Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Sample:
Lane 1: Large intestine (Mouse) Lysate at 40 μg
Lane 2: Small intestine (Mouse) Lysate at 40 μg
Primary: Anti-EpCAM (TMAB-05608) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 38 kD
2. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-TEpCAM antibody (TMAB-05608)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human EpCAM

Human

EPCAM

Epithelial cell adhesion molecule

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.