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Anti-EPAS1 Antibody (7C2)
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Catalog No. TMAC-01821
Alias PASD2, PAS domain-containing protein 2, MOP2, Member of PAS protein 2, Hypoxia-inducible factor 2-alpha, HLF, HIF2-alpha, HIF-2-alpha, HIF2A, HIF-1-alpha-like factor, EPAS-1, Endothelial PAS domain-containing protein 1, Class E basic helix-loop-helix protein 73, bHLHe73, Basic-helix-loop-helix-PAS protein MOP2
Anti-EPAS1 Antibody (7C2) is a Rabbit antibody targeting EPAS1. Anti-EPAS1 Antibody (7C2) can be used in WB,IHC,FCM.
Anti-EPAS1 Antibody (7C2)
Anti-EPAS1 Antibody (7C2)
😃Good
Catalog No. TMAC-01821Alias PASD2, PAS domain-containing protein 2, MOP2, Member of PAS protein 2, Hypoxia-inducible factor 2-alpha, HLF, HIF2-alpha, HIF-2-alpha, HIF2A, HIF-1-alpha-like factor, EPAS-1, Endothelial PAS domain-containing protein 1, Class E basic helix-loop-helix protein 73, bHLHe73, Basic-helix-loop-helix-PAS protein MOP2
Anti-EPAS1 Antibody (7C2) is a Rabbit antibody targeting EPAS1. Anti-EPAS1 Antibody (7C2) can be used in WB,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-EPAS1 Antibody (7C2) is a Rabbit antibody targeting EPAS1. Anti-EPAS1 Antibody (7C2) can be used in WB,IHC,FCM. |
| Synonyms | PASD2, PAS domain-containing protein 2, MOP2, Member of PAS protein 2, Hypoxia-inducible factor 2-alpha, HLF, HIF2-alpha, HIF-2-alpha, HIF2A, HIF-1-alpha-like factor, EPAS-1, Endothelial PAS domain-containing protein 1, Class E basic helix-loop-helix protein 73, bHLHe73, Basic-helix-loop-helix-PAS protein MOP2 |
| Ig Type | IgG |
| Clone | 7C2 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of HUVEC cells with HIF-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 96 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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