Anti-EP4 Polyclonal Antibody 2 is a Rabbit antibody targeting EP4. Anti-EP4 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Mouse

1. Sample:
Lane 1: Mouse Uterus tissue lysates
Lane 2: Mouse Small intestine tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Prostate tissue lysates
Primary: Anti-EP4 (TMAB-05599) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 55 kD
2. Blank control: Ctll-2.
Primary Antibody (green line): Rabbit Anti-EP4 antibody (TMAB-05599)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse EP4

Mouse

PTGER4

Prostaglandin E2 receptor EP4 subtype

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.