Anti-ENPP3 Polyclonal Antibody is a Rabbit antibody targeting ENPP3. Anti-ENPP3 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow)

1. Blank control: Raji (blue). Primary Antibody: Rabbit Anti-ENPP3 antibody (TMAB-05586), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (TMAB-05586, 1 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
2. Black line : Positive blank control (Raji); Negative blank control (Molt4)
Green line : Primary Antibody (Rabbit Anti-ENPP3 antibody (TMAB-05586))
Orange line：Isotype Control Antibody (Rabbit IgG).
Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/Goat anti-rabbit IgG-AF488)
Raji (Positive) and Molt4 (Negative control) Cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ENPP3 antibody (TMAB-05586) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibodY (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ENPP3

Human

ENPP3

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.