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Anti-ENPP3 Antibody (1F307)

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Catalog No. TMAB-05587

Anti-ENPP3 Antibody (1F307) is a Rabbit antibody targeting ENPP3. Anti-ENPP3 Antibody (1F307) can be used in IF, IHC-Fr, IHC-P, WB.

Anti-ENPP3 Antibody (1F307)

Anti-ENPP3 Antibody (1F307)

Copy Product Info
😃Good
Catalog No. TMAB-05587
Anti-ENPP3 Antibody (1F307) is a Rabbit antibody targeting ENPP3. Anti-ENPP3 Antibody (1F307) can be used in IF, IHC-Fr, IHC-P, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ENPP3 Antibody (1F307) is a Rabbit antibody targeting ENPP3. Anti-ENPP3 Antibody (1F307) can be used in IF, IHC-Fr, IHC-P, WB.
Ig Type
IgG
Clone
1F307
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ENPP3 Monoclonal Antibody, Unconjugated (TMAB-05587) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ENPP3 Monoclonal Antibody, Unconjugated (TMAB-05587) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ENPP3 Monoclonal Antibody, Unconjugated (TMAB-05587) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ENPP3 Monoclonal Antibody, Unconjugated (TMAB-05587) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ENPP3 Monoclonal Antibody, Unconjugated (TMAB-05587) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ENPP3 Monoclonal Antibody, Unconjugated (TMAB-05587) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. 25 ug total protein per lane of various lysates (see on figure) probed with ENPP3 monoclonal antibody, unconjugated (TMAB-05587) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
8. 25 ug total protein per lane of various lysates (see on figure) probed with ENPP3 monoclonal antibody, unconjugated (TMAB-05587) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type II membrane protein (Potential). Secreted. Note=Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
Tissue SpecificityExpressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells.
Antigen Details
Immunogen
Recombinant Protein: human ENPP3 protein
Antigen Species
Human
Gene Name
ENPP3
Gene ID
Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Uniprot ID
Function
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
Chemical Properties
Molecular WeightTheoretical: 100 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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