Anti-ENO3 Polyclonal Antibody is a Rabbit antibody targeting ENO3. Anti-ENO3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ENO3 Polyclonal Antibody, Unconjugated (TMAB-05575) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-ENO3 antibody (TMAB-05575)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Pancreas tissue lysates
Lane 4: Rat Liver tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human A549 cell lysates
Lane 9: Human Jurkat cell lysates
Primary: Anti-ENO3 (TMAB-05575) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test'

Polyclonal

KLH conjugated synthetic peptide: human ENO3

Human

ENO3

beta-enolase

Appears to have a function in striated muscle development and regeneration.