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Anti-ENO3 Antibody (6E160)

😃Good
Catalog No. TMAY-02709
Alias MSE, GSD13, enolase 3 (β, muscle), enolase 3 (beta, muscle)

Anti-ENO3 Antibody (6E160) is a Rabbit antibody targeting ENO3. Anti-ENO3 Antibody (6E160) can be used in WB,IHC-P,IP.

Anti-ENO3 Antibody (6E160)

Anti-ENO3 Antibody (6E160)

😃Good
Catalog No. TMAY-02709Alias MSE, GSD13, enolase 3 (β, muscle), enolase 3 (beta, muscle)
Anti-ENO3 Antibody (6E160) is a Rabbit antibody targeting ENO3. Anti-ENO3 Antibody (6E160) can be used in WB,IHC-P,IP.
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100 μL256 € 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ENO3 Antibody (6E160) is a Rabbit antibody targeting ENO3. Anti-ENO3 Antibody (6E160) can be used in WB,IHC-P,IP.
AliasMSE, GSD13, enolase 3 (β, muscle), enolase 3 (beta, muscle)
Ig Type
Monoclonal Rabbit IgG
Clone
6E160
Reactivity
Human
Verified Activity
1. Immunochemical staining of human ENO3 in human skeletal muscle with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

2. Anti-ENO3 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: Raji Whole Cell Lysate.

-Lane C: Hela Whole Cell Lysate.

-Lane D: MOLT-4 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:47 kDa.

-Observed band size:50 kDa.

3. ENO3 was immunoprecipitated using:

-Lane A:0.5 mg Raji Whole Cell Lysate.

-2 µL anti-ENO3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-ENO3 rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution.

-Developed using the DAB staining technique.

-Performed under reducing conditions.

-Predicted band size: 47 kDa.

-Observed band size: 53 kDa
Application
WB,IHC-P,IP
Recommended Dose
WB: 1:500-1:2000; IHC-P: 1:100-1:500; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; TMPY-03446; AAH17249.1; Met1-Lys434).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ENO3 / beta-enolase Protein (TMPY-03446)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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