Anti-ENO1 Polyclonal Antibody is a Rabbit antibody targeting ENO1. Anti-ENO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Cow,Rabbit)

1. Tissue/cell: Rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ENO1 Polyclonal Antibody, Unconjugated (TMAB-00614) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. 25 μg total protein per Lane of various lysates probed with ENO1 polyclonal antibody, unconjugated (TMAB-00614) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ENO1

Human

Metabolism of carbohydrates,Tumor suppressors,Tumor Suppressors,Co-factors,Other factors,Transcription Factors,Co-factors,Carbohydrate metabolism,Energy Metabolism,Energy Metabolism