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Anti-ENO1 Polyclonal Antibody
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Catalog No. TMAB-00614
Alias PPH, NNE, MPB1, HEL-S-17, enolase 1, (α), enolase 1, (alpha), ENO1L1
Anti-ENO1 Polyclonal Antibody is a Rabbit antibody targeting ENO1. Anti-ENO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ENO1 Polyclonal Antibody
Anti-ENO1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00614Alias PPH, NNE, MPB1, HEL-S-17, enolase 1, (α), enolase 1, (alpha), ENO1L1
Anti-ENO1 Polyclonal Antibody is a Rabbit antibody targeting ENO1. Anti-ENO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ENO1 Polyclonal Antibody is a Rabbit antibody targeting ENO1. Anti-ENO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | PPH, NNE, MPB1, HEL-S-17, enolase 1, (α), enolase 1, (alpha), ENO1L1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Cow,Rabbit) |
| Verified Activity | 1. Tissue/cell: Rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ENO1 Polyclonal Antibody, Unconjugated (TMAB-00614) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 2. 25 μg total protein per Lane of various lysates probed with ENO1 polyclonal antibody, unconjugated (TMAB-00614) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Isoform MBP-1: Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Enolase 1 is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. Enolase 2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Enolase 3 appears to have a function in striated muscle development and regeneration. |
| Immunogen | KLH conjugated synthetic peptide: human ENO1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Metabolism of carbohydrates,Tumor suppressors,Tumor Suppressors,Co-factors,Other factors,Transcription Factors,Co-factors,Carbohydrate metabolism,Energy Metabolism,Energy Metabolism |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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