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Anti-Endothelin-1/EDN1 Polyclonal Antibody (Synonyms: Preproendothelin-1 (PPET1), Endothelin-1, EDN1)
Catalog No. TMAB-00611 Copy Product Info
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Anti-Endothelin-1/EDN1 Polyclonal Antibody is a Rabbit antibody targeting Endothelin-1/EDN1. Anti-Endothelin-1/EDN1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
Anti-Endothelin-1/EDN1 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-00611
Synonyms Preproendothelin-1 (PPET1), Endothelin-1, EDN1
Anti-Endothelin-1/EDN1 Polyclonal Antibody is a Rabbit antibody targeting Endothelin-1/EDN1. Anti-Endothelin-1/EDN1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
Anti-Endothelin-1/EDN1 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Endothelin-1/EDN1 Polyclonal Antibody is a Rabbit antibody targeting Endothelin-1/EDN1. Anti-Endothelin-1/EDN1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB. |
| Synonyms | Preproendothelin-1 (PPET1), Endothelin-1, EDN1 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Big endothelin-1) Polyclonal Antibody, Unconjugated (TMAB-00611) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Big endothelin-1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Big endothelin-1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lane 1: Testis (Mouse) Lysate at 40 μg Lane 2: Testis (Rat) Lysate at 40 μg Primary: Anti-Big endothelin-1 (TMAB-00611) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 26 kDa     |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Molecular weight: 2491.9 Molecular formula: C109H159N25O32S5 CAS: 117399-94-7 MDL: MFCD00133305 Reconstitution: 1mM (1mg/0.4013mL) |
| Immunogen | KLH conjugated synthetic peptide: human Big endothelin-1 |
| Antigen Species | Human |
| Gene Name | EDN1 |
| Gene ID | |
| Protein Name | Endothelin-1 |
| Uniprot ID | |
| Biology Area | Hormones,Cancer,Hypoxia,Vasoconstriction,Endothelium,Response to hypoxia |
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. |
| Molecular Weight | Theoretical: 22 kDa. Actual: 24-27 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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