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Anti-Endostatin Polyclonal Antibody
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Catalog No. TMAB-00610
Alias type XVIII, Endostatin, collagen α-1(XVIII)chain, collagen alpha-1(XVIII)chain, collagen, COL18A1, antiangiogenic agent
Anti-Endostatin Polyclonal Antibody is a Rabbit antibody targeting Endostatin. Anti-Endostatin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Endostatin Polyclonal Antibody
Anti-Endostatin Polyclonal Antibody
😃Good
Catalog No. TMAB-00610Alias type XVIII, Endostatin, collagen α-1(XVIII)chain, collagen alpha-1(XVIII)chain, collagen, COL18A1, antiangiogenic agent
Anti-Endostatin Polyclonal Antibody is a Rabbit antibody targeting Endostatin. Anti-Endostatin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Endostatin Polyclonal Antibody is a Rabbit antibody targeting Endostatin. Anti-Endostatin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | type XVIII, Endostatin, collagen α-1(XVIII)chain, collagen alpha-1(XVIII)chain, collagen, COL18A1, antiangiogenic agent |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-Endostatin (TMAB-00610) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/190 kDa Observed band size: 155 kDa 2. Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Endostatin Polyclonal Antibody, Unconjugated (TMAB-00610) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide: human Endostatin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Angiogenic inhibitory factors,Inhibitors,Collagen |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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