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Anti-EMR4 Polyclonal Antibody

Catalog No. TMAB-05560 Copy Product Info
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Anti-EMR4 Polyclonal Antibody is a Rabbit antibody targeting EMR4. Anti-EMR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-EMR4 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-05560

Anti-EMR4 Polyclonal Antibody is a Rabbit antibody targeting EMR4. Anti-EMR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-EMR4 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-EMR4 Polyclonal Antibody is a Rabbit antibody targeting EMR4. Anti-EMR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Cow,Sheep)
Verified Activity
1. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-EMR4 (TMAB-05560) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
2. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EMR4) Polyclonal Antibody, Unconjugated (TMAB-05560) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EMR4) Polyclonal Antibody, Unconjugated (TMAB-05560) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell Membrane; multi pass membrane protein
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene is a member of the EGF-TM7 receptor gene family which is thought to play a role in leukocyte adhesion and migration. In other vertebrates, including nonhuman primates, this gene encodes a protein containing N-terminal EGF domains and a C-terminal transmembrane domain. Sequence evidence for the human gene, however, indicates nucleotide deletion in the genomic sequence would result in frameshift and early termination of translation. A protein expressed by this gene would be soluble rather than expressed on the cell surface. As the encoded protein has not been detected, this gene may represent a transcribed pseudogene. [provided by RefSeq, Aug 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EMR4
Antigen Species
Human
Gene Name
ADGRE4P
Gene ID
Protein Name
Putative adhesion G protein-coupled receptor E4P
Uniprot ID
Function
EMR4 could mediate the cellular interaction between myeloid cells and B-cells.
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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