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Anti-Emi1 Antibody (9H429)
😃Good
Catalog No. TMAC-01288
Alias fbxo5, Fbxo31, FBX5_HUMAN, FBX5, F-box only protein 5, F box protein Fbx5, F box protein Fbx 5, F box protein 5, F box only protein 5, EMI1, Emi 1, Early mitotic inhibitor 1
Anti-Emi1 Antibody (9H429) is a Rabbit antibody targeting Emi1. Anti-Emi1 Antibody (9H429) can be used in ICC,IHC,WB.
Anti-Emi1 Antibody (9H429)
Anti-Emi1 Antibody (9H429)
😃Good
Catalog No. TMAC-01288Alias fbxo5, Fbxo31, FBX5_HUMAN, FBX5, F-box only protein 5, F box protein Fbx5, F box protein Fbx 5, F box protein 5, F box only protein 5, EMI1, Emi 1, Early mitotic inhibitor 1
Anti-Emi1 Antibody (9H429) is a Rabbit antibody targeting Emi1. Anti-Emi1 Antibody (9H429) can be used in ICC,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Emi1 Antibody (9H429) is a Rabbit antibody targeting Emi1. Anti-Emi1 Antibody (9H429) can be used in ICC,IHC,WB. |
| Synonyms | fbxo5, Fbxo31, FBX5_HUMAN, FBX5, F-box only protein 5, F box protein Fbx5, F box protein Fbx 5, F box protein 5, F box only protein 5, EMI1, Emi 1, Early mitotic inhibitor 1 |
| Ig Type | IgG |
| Clone | 9H429 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 6. ICC staining Emi1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Emi1 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.        |
| Application | |
| Recommended Dose | IHC: 1:50-200; ICC: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human Emi1 aa 50-250 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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