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Anti-Elastin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00605
Anti-Elastin Polyclonal Antibody 2 is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Elastin Polyclonal Antibody 2
Anti-Elastin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00605
Anti-Elastin Polyclonal Antibody 2 is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. The figure annotation: The blue histogram is unstained cells. The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (TMAB-00605) plus secondary antibody. Controls: Positive control: A549 cells. Isotype control: Cell lines treated with rabbit IgG at 1:1000 4°C temperature overnight 4°Cfollowed by conjugation to secondary antibody. 3. Sample: Lung (Mouse) Lysate at 30 μg Primary: Anti-alpha Elastin/Tropoelastin (TMAB-00605) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 70 kDa Observed band size: 68 kDa 4. Sample: Lane 1: Uterus (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: Liver (Mouse) Lysate at 40 μg Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 5: Lung (Rat) Lysate at 40 μg Lane 6: Liver (Rat) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 9: A549 (Human) Cell Lysate at 30 μg Primary: Anti-Elastin (TMAB-00605) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 68 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017]. |
| Immunogen | KLH conjugated synthetic peptide: human Elastin |
| Antigen Species | Human |
| Gene ID | 2006 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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