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Anti-eIF4ENIF1 Polyclonal Antibody
Catalog No. TMAB-05522 Copy Product Info
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Anti-eIF4ENIF1 Polyclonal Antibody is a Rabbit antibody targeting eIF4ENIF1. Anti-eIF4ENIF1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-eIF4ENIF1 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-05522
Anti-eIF4ENIF1 Polyclonal Antibody is a Rabbit antibody targeting eIF4ENIF1. Anti-eIF4ENIF1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-eIF4ENIF1 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-eIF4ENIF1 Polyclonal Antibody is a Rabbit antibody targeting eIF4ENIF1. Anti-eIF4ENIF1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Cow,Horse,Rabbit,Turkey,Pig,Chimpanzee,Rhesus monkey,Gori) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eIF4ENIF1) Polyclonal Antibody, Unconjugated (TMAB-05522) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eIF4ENIF1) Polyclonal Antibody, Unconjugated (TMAB-05522) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eIF4ENIF1) Polyclonal Antibody, Unconjugated (TMAB-05522) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-eIF4ENIF1 Polyclonal Antibody, Unconjugated (TMAB-05522) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. |
| Tissue Specificity | Widely expressed. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
| Immunogen | KLH conjugated synthetic peptide: human eIF4ENIF1 |
| Antigen Species | Human |
| Gene Name | EIF4ENIF1 |
| Gene ID | |
| Protein Name | Eukaryotic translation initiation factor 4E transporter |
| Uniprot ID | |
| Function | Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. |
| Molecular Weight | Theoretical: 108 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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