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Anti-EIF4E2 Polyclonal Antibody

Catalog No. TMAB-05519 Copy Product Info
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Anti-EIF4E2 Polyclonal Antibody is a Rabbit antibody targeting EIF4E2. Anti-EIF4E2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-EIF4E2 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-05519

Anti-EIF4E2 Polyclonal Antibody is a Rabbit antibody targeting EIF4E2. Anti-EIF4E2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-EIF4E2 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-EIF4E2 Polyclonal Antibody is a Rabbit antibody targeting EIF4E2. Anti-EIF4E2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-EIF4EL3 Polyclonal Antibody, Unconjugated (TMAB-05519) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation factor 4E family (eIF4E) is comprised of three proteins that are involved in the early initiation of protein synthesis. eIF4E2 (eukaryotic translation initiation factor 4E family member 2), also known as 4EHP, IF4e, 4E-LP or EIF4EL3, is a ubiquitously expressed 245 amino acid protein. During early translation events, eIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap (a cotranscriptionally added structure that conveys mRNA stability and allows for efficient RNA processing), thus initiating the unwinding of mRNA secondary structures and facilitating mRNA-ribosome binding. eIF4E2 competes with eIF4E (member 1) for cap binding and, upon modification by the ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein), exhibits increased mRNA cap affinity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EIF4EL3
Antigen Species
Human
Gene Name
EIF4E2
Gene ID
Protein Name
Eukaryotic translation initiation factor 4E type 2
Uniprot ID
Function
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Chemical Properties
Molecular WeightTheoretical: 28 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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