Anti-eIF4A1 Antibody (8D113) is a Rabbit antibody targeting eIF4A1. Anti-eIF4A1 Antibody (8D113) can be used in WB,ICC/IF.

IgG

8D113

Human,Mouse (predicted:Rat)

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (eIF4A1) monoclonal Antibody, unconjugated (TMAB-05512) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with eIF4A1 monoclonal antibody, unconjugated (TMAB-05512) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human eIF4A1

Human

EIF4A1

Eukaryotic initiation factor 4A-I

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.