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Anti-EIF4A1 Antibody (4N444)
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Catalog No. TMAC-01271
Anti-EIF4A1 Antibody (4N444) is a Mouse antibody targeting EIF4A1. Anti-EIF4A1 Antibody (4N444) can be used in WB,IHC,IF.
Anti-EIF4A1 Antibody (4N444)
Anti-EIF4A1 Antibody (4N444)
😃Good
Catalog No. TMAC-01271
Anti-EIF4A1 Antibody (4N444) is a Mouse antibody targeting EIF4A1. Anti-EIF4A1 Antibody (4N444) can be used in WB,IHC,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-EIF4A1 Antibody (4N444) is a Mouse antibody targeting EIF4A1. Anti-EIF4A1 Antibody (4N444) can be used in WB,IHC,IF. |
| Clone | 4N444 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue, using TMAC-01271 diluted at 1:2,000. 2. Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 3. Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 4. Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 5. IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100. 6. Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B. |
| Application | |
| Recommended Dose | WB: 1:1000-3000; IF: 1:100-200; IHC: 1:50-300 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Appearance | Liquid |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research Background | The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA ¨C this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression. |
| Conjucates | Unconjugated |
| Uniprot ID |
| Molecular Weight | Actual: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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