Anti-EIF3M Antibody (9N524) is a Mouse antibody targeting EIF3M. Anti-EIF3M Antibody (9N524) can be used in WB,ICC/IF.

IgG2a, Kappa

9N524

Human (predicted:Mouse,Rat)

1. Cell line: MCF-7
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-05506
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HepG2, PC-3, PC-12, EL4.IL-2
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 43 kDa
Observed MW: 43 kDa

WB: 1:500-1000; ICC/IF: 1:100-500

Monoclonal

EIF3M

Eukaryotic translation initiation factor 3 subunit M

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).