Anti-EIF2AK4/GCN2 Antibody (1T579) is a Rabbit antibody targeting EIF2AK4/GCN2. Anti-EIF2AK4/GCN2 Antibody (1T579) can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

1T579

Human

1. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-EIF2AK4/GCN2 antibody (TMAB-05491): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (EIF2AK4/GCN2) monoclonal Antibody, unconjugated (TMAB-05491) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human GCN2

Human

EIF2AK4

eIF-2-alpha kinase GCN2

Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.