Anti-EIF2AK2 Antibody (9R676) is a Mouse antibody targeting EIF2AK2. Anti-EIF2AK2 Antibody (9R676) can be used in WB,ICC/IF.

IgG1, k

9R676

Human

1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EIF2AK2) monoclonal Antibody, Unconjugated (TMAB-05488) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, Alexa Fluor® 555 conjugated with Phalloidin (red) was used to stain the cell Cytoplasmic actin. The nuclear counter stain is DAPI (blue)
2. Sample:
Lane 1: A431 cell lysates
Lane 2: HepG2 cell lysates
Lane 3: SH-SY5Y cell lysates
Primary: Anti-EIF2AK2 (TMAB-05488) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 69 kD

WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: human EIF2AK2

Human

EIF2AK2

Interferon-induced, double-stranded RNA-activated protein kinase

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.