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Anti-eIF2A Antibody (9O14)
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Catalog No. TMAB-05486
Anti-eIF2A Antibody (9O14) is a Rabbit antibody targeting eIF2A. Anti-eIF2A Antibody (9O14) can be used in FCM, ICC/IF, WB.
Anti-eIF2A Antibody (9O14)
Anti-eIF2A Antibody (9O14)
Copy Product Info😃Good
Catalog No. TMAB-05486
Anti-eIF2A Antibody (9O14) is a Rabbit antibody targeting eIF2A. Anti-eIF2A Antibody (9O14) can be used in FCM, ICC/IF, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-eIF2A Antibody (9O14) is a Rabbit antibody targeting eIF2A. Anti-eIF2A Antibody (9O14) can be used in FCM, ICC/IF, WB. |
| Ig Type | IgG |
| Clone | 9O14 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 ug total protein per lane of various lysates (see on figure) probed with eIF2A monoclonal antibody, unconjugated (TMAB-05486) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The U-87MG (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-eIF2A antibody (TMAB-05486, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM=1:50-100; ICC/IF=1:50-200; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. |
| Immunogen | A synthesized peptide: human eIF2A |
| Antigen Species | Human |
| Gene Name | EIF2A |
| Gene ID | |
| Protein Name | Eukaryotic translation initiation factor 2A |
| Uniprot ID |
| Molecular Weight | Theoretical: 65 kDa. Actual: 65 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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