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Anti-EGLN2 Antibody (7L776)
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Catalog No. TMAC-03183
Alias Prolyl hydroxylase EGLN2, Prolyl hydroxylase domain-containing protein 1 (PHD1), Hypoxia-inducible factor prolyl hydroxylase 1 (HIF-PH1, HPH-3, HPH-1), HIF-prolyl hydroxylase 1, Estrogen-induced tag 6 (EIT-6), EIT6, EGLN2, Egl nine homolog 2
Anti-EGLN2 Antibody (7L776) is a Rabbit antibody targeting EGLN2. Anti-EGLN2 Antibody (7L776) can be used in WB,ICC/IF,IHC,FCM.
Anti-EGLN2 Antibody (7L776)
Anti-EGLN2 Antibody (7L776)
😃Good
Catalog No. TMAC-03183Alias Prolyl hydroxylase EGLN2, Prolyl hydroxylase domain-containing protein 1 (PHD1), Hypoxia-inducible factor prolyl hydroxylase 1 (HIF-PH1, HPH-3, HPH-1), HIF-prolyl hydroxylase 1, Estrogen-induced tag 6 (EIT-6), EIT6, EGLN2, Egl nine homolog 2
Anti-EGLN2 Antibody (7L776) is a Rabbit antibody targeting EGLN2. Anti-EGLN2 Antibody (7L776) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-EGLN2 Antibody (7L776) is a Rabbit antibody targeting EGLN2. Anti-EGLN2 Antibody (7L776) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Prolyl hydroxylase EGLN2, Prolyl hydroxylase domain-containing protein 1 (PHD1), Hypoxia-inducible factor prolyl hydroxylase 1 (HIF-PH1, HPH-3, HPH-1), HIF-prolyl hydroxylase 1, Estrogen-induced tag 6 (EIT-6), EIT6, EGLN2, Egl nine homolog 2 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: PC12, Lane 3: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin. 6. ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-α subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-α proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3. HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1α in normoxia. HIF PHD2, a direct HIF target gene, is expressed mainly in skeletal muscle, heart, kidney and brain. HIF PHD3 may play a role in the regulation of cell growth in muscle cells and in apoptosis in neuronal tissue. HIF PHD3 is widely expressed, although the highest levels can be detected in placenta and he |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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